normal cervical epithelial cell line ect1 e6e7  (ATCC)

Journal: Cancer Immunology, Immunotherapy

Article Title: Oct4 activates IL-17A to orchestrate M2 macrophage polarization and cervical cancer metastasis

doi: 10.1007/s00262-023-03596-z

Figure Lengend Snippet: IL-17A induces M2 macrophage polarization in cervical cancer. A , B RT-qPCR was used to measure IL-17A gene expression level in adjacent normal tissues, cervical cancer tissues, primary tumor tissues, and metastatic tumor tissues. C RT-qPCR and ELISA outcomes of IL-17A expression in ende1617, HeLa, and SiHa cells. D THP1 cells (treated with PMA) were treated with IL-17A (50 ng/mL) or PBS. RT-qPCR was utilized to measure the gene expression levels of CD206, CD163, CD86, and CD80 expression. E The diagrammatic sketch of cell co-culture system. F RT-qPCR was utilized to measure the gene expression levels of M1 macrophage markers (CD86, CD80, Tnf1, Ros1) and M2 macrophage markers (CD206, CD163, Veg1, Tgfβ). G , H Surface expression of CD86 and CD206 was detected in THP-1 (PMA) + Hela(sh-OCT4-1) cells using flow cytometry. The histograms represent the percent of CD86 or CD206 cells in THP-1 (PMA) + Hela(sh-OCT4-1) cells. * p < 0.05; ** p < 0.01; *** p < 0.001. All experiments were repeated at least three times

Article Snippet: Cervical cancer cell lines HeLa and SiHa, normal cervical epithelial cell line ende1617, and human monocytic cell line THP-1 all were purchased from ATCC (Manassas, VA).

Techniques: Quantitative RT-PCR, Gene Expression, Enzyme-linked Immunosorbent Assay, Expressing, Co-Culture Assay, Flow Cytometry